Cholinergic innervation and ganglion cell distribution in Hirschsprung's disease.

ABSTRACT:

Background

The diagnostic gold standard of Hirschsprung's disease (HD) is based on the histopathological assessment of colorectal biopsies. Although data on cholinergic innervation and ganglion cell (GC) distribution exist, only few studies have examined these two key features together. We assessed the pattern of cholinergic innervation and the amount of GCs in colorectal specimens of 14 HD patients.

Methods

We established a semi-quantitative score for cholinergic innervation using acetylcholinesterase (AChE) enzyme histochemistry and quantitatively analyzed the number of GCs via NADH tetrazolium reductase (NADH) enzyme histochemistry. We examined both the entire length of the resected specimens as well as defined areas of the transition zone of both pathological and healthy

SUBMITTER: Braczynski AK 

PROVIDER: S-EPMC7445925 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature