Upregulation of METTL14 mediates the elevation of PERP mRNA N⁶ adenosine methylation promoting the growth and metastasis of pancreatic cancer.

ABSTRACT:

Background

Pancreatic cancer is one of the most lethal human cancers. N⁶-methyladenosine (m⁶A), a common eukaryotic mRNA modification, plays critical roles in both physiological and pathological processes. However, its role in pancreatic cancer remains elusive.

Methods

LC/MS was used to profile m⁶A levels in pancreatic cancer and normal tissues. Bioinformatics analysis, real-time PCR, immunohistochemistry, and western blotting were used to identify the role of m⁶A regulators in pancreatic cancer. The biological effects of methyltransferase-like 14 (METTL14), an mRNA methylase, were investigated using in vitro and in vivo models. MeRIP-Seq and RNA-Seq were used to assess the downstream targets of METTL14.

Results

We found tha

SUBMITTER: Wang M 

PROVIDER: S-EPMC7446161 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature