CREB5 promotes invasiveness and metastasis in colorectal cancer by directly activating MET.

ABSTRACT:

Background

cAMP responsive element binding protein 5 (CREB5) is a transcriptional activator in eukaryotic cells that can regulate gene expression. Previously, we found that CREB5 was involved in the occurrence and development of colorectal cancer (CRC) using bioinformatics analysis. However, the biological roles and underlying regulatory mechanism of CREB5 in CRC remain unclear.

Methods

Real-time PCR, western blotting, and immunohistochemistry were used to examine CREB5 expression. In vitro experiments including migration assay, wound-healing assay, chicken chorioallantoic membrane assay, and human umbilical vein endothelial cells tube formation assay were used to investigate the effects of CREB5 on CRC cell migration and tumor angiogenesis ability. Additionally, an orthotop

SUBMITTER: Wang S 

PROVIDER: S-EPMC7446182 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature