Altered IL-32 Signaling in Abdominal Aortic Aneurysm.

ABSTRACT: INTRODUCTION AND OBJECTIVE:Interleukin (IL)-32 is a pro-inflammatory cytokine not previously studied in relation to abdominal aortic aneurysm (AAA). The aim of this study was to elucidate the expression and localization of IL-32 in AAA. METHODS:Expression and localization of IL-32 in human aortic tissue was studied with immunohistochemical analysis and Western blot (AAA: n = 5; controls: n = 4). ELISA was used to measure IL-32 in human plasma samples (AAA: n = 140; controls: n = 37) and in media from cultured peripheral blood mononuclear cells (PBMCs) from 3 healthy donors. IL-32 mRNA in PBMCs, endothelial cells, aortic smooth muscle cells (SMCs), and aortic tissue samples of AAA (n = 16) and control aortas (n = 9) was measured with qPCR. RESULTS:IL-32 was predominantly expressed in SMCs a

SUBMITTER: Bengts S 

PROVIDER: S-EPMC7446302 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature