Neutrophil Extracellular Trap Degradation by Differently Polarized Macrophage Subsets.

ABSTRACT:

Objective

Macrophages are immune cells, capable to remodel the extracellular matrix, which can harbor extracellular DNA incorporated into neutrophil extracellular traps (NETs). To study the breakdown of NETs we studied the capability of macrophage subsets to degrade these structures in vitro and in vivo in a murine thrombosis model. Furthermore, we analyzed human abdominal aortic aneurysm samples in support of our in vitro and in vivo results. Approach and Results: Macrophages were seeded onto blood clots or isolated NETs and polarized. All macrophages were capable to degrade NETs. For initial breakdown, macrophages relied on extracellular deoxyribonucleases. Proinflammatory polarization enhanced NET degradation. The boost in degradation was because of increased macropinocytosis, a

SUBMITTER: Haider P 

PROVIDER: S-EPMC7447175 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature