MiRNA-425-5p enhances lung cancer growth via the PTEN/PI3K/AKT signaling axis.

ABSTRACT:

Background

miRNAs regulate a multitude of cellular processes and their aberrant regulation is linked to human cancer. However, the role of miR-425-5p in lung cancer (LCa) is still largely unclear. Here, we explored the role of miR-425-5p during LCa tumorigenesis.

Methods

Cell proliferation was evaluated by cell counting Kit-8 and colony formation assay. Western blot and real-time PCR were accordingly used to detect the relevant proteins, miRNA and gene expression. Luciferase reporter assays were used to illustrate the interaction between miR-425-5p and PTEN.

Results

We demonstrate that miR-425-5p is overexpressed in LCa tissue and enhances the proliferative and colony formation capacity of the LCa cell lines A549 and NCI-H1299. Through predictive binding assays, PTEN

SUBMITTER: Zhou JS 

PROVIDER: S-EPMC7447575 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature