Vendor effects on murine gut microbiota and its influence on lipopolysaccharide-induced lung inflammation and Gram-negative pneumonia.

ABSTRACT:

Background

The microbiome has emerged as an important player in the pathophysiology of a whole spectrum of diseases that affect the critically ill. We hypothesized that differences in microbiota composition across vendors can influence murine models of pulmonary lipopolysaccharide (LPS) inflammation and Gram-negative pneumonia.

Methods

A multi-vendor approach was used with genetically similar mice derived from three different vendors (Janvier, Envigo, Charles River). This model was employed to study the effect on the host response to a pulmonary LPS challenge (1 μg Klebsiella pneumoniae LPS, intranasal), as well as experimental K. pneumoniae infection (ATCC43816, 1 × 10⁴ CFU, intranasal).

Results

Gut microbiota analysis revealed profound intervendor differe

SUBMITTER: Wolff NS 

PROVIDER: S-EPMC7447702 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature