Characterization of FLT3-ITD^mut acute myeloid leukemia: molecular profiling of leukemic precursor cells.

ABSTRACT: Acute myeloid leukemia (AML) with FLT3-ITD mutations (FLT3-ITD^mut) remains a therapeutic challenge, with a still high relapse rate, despite targeted treatment with tyrosine kinase inhibitors. In this disease, the CD34/CD123/CD25/CD99+ leukemic precursor cells (LPCs) phenotype predicts for FLT3-ITD-positivity. The aim of this study was to characterize the distribution of FLT3-ITD mutation in different progenitor cell subsets to shed light on the subclonal architecture of FLT3-ITD^mut AML. Using high-speed cell sorting, we sequentially purified LPCs and CD34+ progenitors in samples from patients with FLT3-ITD^mut AML (n = 12). A higher FLT3-ITD^mut load was observed within CD34/CD123/CD25/CD99+ LPCs, as compared to CD34+ progenitors (CD123+/-,CD25-,CD

SUBMITTER: Travaglini S 

PROVIDER: S-EPMC7447750 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature