Online monitoring of the respiratory quotient reveals metabolic phases during microaerobic 2,3-butanediol production with Bacillus licheniformis.

ABSTRACT: Microaerobic cultivation conditions are often beneficial for the biotechnological production of reduced metabolites like 2,3-butanediol. However, due to oxygen limitation, process monitoring based on oxygen transfer rate, or dissolved oxygen measurement provides only limited information. In this study, online monitoring of the respiratory quotient is used to investigate the metabolic activity of Bacillus licheniformis DSM 8785 during mixed acid-2,3-butanediol production under microaerobic conditions. Thereby, the respiratory quotient provides valuable information about different metabolic phases. Based on partial reaction stoichiometries, the metabolic activity in each phase of the cultivation was revealed, explaining the course of the respiratory quotient. This provides profound in

SUBMITTER: Heyman B 

PROVIDER: S-EPMC7447875 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature