Ontology highlight
ABSTRACT:
SUBMITTER: Hinch AG
PROVIDER: S-EPMC7447979 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Hinch Anjali Gupta AG Becker Philipp W PW Li Tao T Moralli Daniela D Zhang Gang G Bycroft Clare C Green Catherine C Keeney Scott S Shi Qinghua Q Davies Benjamin B Donnelly Peter P
Molecular cell 20200630 4
Meiotic recombination proceeds via binding of RPA, RAD51, and DMC1 to single-stranded DNA (ssDNA) substrates created after formation of programmed DNA double-strand breaks. Here we report high-resolution in vivo maps of RPA and RAD51 in meiosis, mapping their binding locations and lifespans to individual homologous chromosomes using a genetically engineered hybrid mouse. Together with high-resolution microscopy and DMC1 binding maps, we show that DMC1 and RAD51 have distinct spatial localization ...[more]