Dataset Information

Improved reference genome of the arboviral vector Aedes albopictus.

ABSTRACT:

Background

The Asian tiger mosquito Aedes albopictus is globally expanding and has become the main vector for human arboviruses in Europe. With limited antiviral drugs and vaccines available, vector control is the primary approach to prevent mosquito-borne diseases. A reliable and accurate DNA sequence of the Ae. albopictus genome is essential to develop new approaches that involve genetic manipulation of mosquitoes.

Results

We use long-read sequencing methods and modern scaffolding techniques (PacBio, 10X, and Hi-C) to produce AalbF2, a dramatically improved assembly of the Ae. albopictus genome. AalbF2 reveals widespread viral insertions, novel microRNAs and piRNA clusters, the sex-determining locus, and new immunity genes, and enables genome-wide studies of geographically diverse Ae. albopictus populations and analyses of the developmental and stage-dependent network of expression data. Additionally, we build the first physical map for this species with 75% of the assembled genome anchored to the chromosomes.

Conclusion

The AalbF2 genome assembly represents the most up-to-date collective knowledge of the Ae. albopictus genome. These resources represent a foundation to improve understanding of the adaptation potential and the epidemiological relevance of this species and foster the development of innovative control measures.

SUBMITTER: Palatini U

PROVIDER: S-EPMC7448346 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature

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Publications

Improved reference genome of the arboviral vector Aedes albopictus.

Palatini Umberto U Masri Reem A RA Cosme Luciano V LV Koren Sergey S Thibaud-Nissen Françoise F Biedler James K JK Krsticevic Flavia F Johnston J Spencer JS Halbach Rebecca R Crawford Jacob E JE Antoshechkin Igor I Failloux Anna-Bella AB Pischedda Elisa E Marconcini Michele M Ghurye Jay J Rhie Arang A Sharma Atashi A Karagodin Dmitry A DA Jenrette Jeremy J Gamez Stephanie S Miesen Pascal P Masterson Patrick P Caccone Adalgisa A Sharakhova Maria V MV Tu Zhijian Z Papathanos Philippos A PA Van Rij Ronald P RP Akbari Omar S OS Powell Jeffrey J Phillippy Adam M AM Bonizzoni Mariangela M

Genome biology 20200826 1

<h4>Background</h4>The Asian tiger mosquito Aedes albopictus is globally expanding and has become the main vector for human arboviruses in Europe. With limited antiviral drugs and vaccines available, vector control is the primary approach to prevent mosquito-borne diseases. A reliable and accurate DNA sequence of the Ae. albopictus genome is essential to develop new approaches that involve genetic manipulation of mosquitoes.<h4>Results</h4>We use long-read sequencing methods and modern scaffoldi ...[more]

PMID: 32847630

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Project description:In the last four decades there has been a staggering increase in the geographical range of the arboviral vector Aedes albopictus (Skuse, 1894). This species is now found in every continent except Antarctica, increasing the distribution of arboviral diseases such as dengue and chikungunya. In Lao PDR dengue epidemics occur regularly, with cases of chikungunya also reported. As treatment methods for arboviral diseases is limited, the control of the vector mosquitoes are essential. There is a paucity of information on the bionomics and resistance status of this mosquito for successful vector control efforts. Here we describe the bionomics and insecticide resistance status of Ae. albopictus in Laos to identify opportunities for control. Adult Ae. albopictus were collected using human-baited double bed net (HDN) traps in forests, villages and rubber plantations and tested for alpha- and flaviviruses with RT-PCR. Surveys were also conducted to identify larval habitats. Seven adult and larval populations originating from Vientiane Capital and Luang Prabang province were tested against DDT, malathion, permethrin, deltamethrin and, temephos following WHO protocols. Aedes albopictus were found throughout the year, but were six-fold greater in the rainy season than the dry season. Adult females were active for 24 hours, with peak of behaviour at 18.00 h. The secondary forest and rubber plantation samples showed evidence of Pan-flaviviruses, while samples from the villages did not. More than half of the emerged Ae. albopictus were collected from mature rubber plantations (53.9%; 1,533/2,845). Most Ae. albopictus mosquitoes emerged from latex collection cups (19.7%; 562/2,845), small water containers (19.7%; 562/2,845) and tyres (17.4%; 495/2,845). Adult mosquitoes were susceptible to pyrethroids, apart from one population in Vientiane city. All populations were resistant to DDT (between 27-90% mortality) and all except one were resistant to malathion (20-86%). Three of the seven larval populations were resistant to temephos (42-87%), with suspected resistance found in three other populations (92-98%).This study demonstrates that rural areas in northern Laos are potential hot spots for arboviral disease transmission. Multiple-insecticide resistance was found. Aedes albopictus control efforts in villages need to expand to include secondary forests and rubber plantations, with larval source management and limited use of insecticides.

Project description:BackgroundThe arboviral vector Aedes albopictus became established on all continents except Antarctica in the past 50 years. A consequence of its rapid global invasion is the transmission of diseases previously confined to the tropics and subtropics occurring in temperate regions of the world, including the re-emergence of chikungunya and dengue in Europe. Application of pyrethroids is among the most widely-used interventions for vector control, especially in the presence of an arboviral outbreak. Studies are emerging that reveal phenotypic resistance and monitor mutations at the target site, the para sodium channel gene, primarily on a local scale.MethodsA total of 512 Ae. albopictus mosquitoes from twelve geographic sites, including those from the native home range and invaded areas, were sampled between 2011 and 2018, and were analyzed at five codons of the para sodium channel gene with mutations predictive of resistance phenotype. Additionally, to test for the origin of unique kdr mutations in Mexico, we analyzed the genetic connectivity of southern Mexico mosquitoes with mosquitoes from home range, the Reunion Island, America and Europe.ResultsWe detected mutations at all tested positions of the para sodium channel gene, with heterozygotes predominating and rare instance of double mutants. We observed an increase in the distribution and frequency of F1534C/L/S mutations in the ancestral China population and populations in the Mediterranean Greece, the appearance of the V1016G/I mutations as early as 2011 in Italy and mutations at position 410 and 989 in Mexico. The analyses of the distribution pattern of kdr alleles and haplotype network analyses showed evidence for multiple origins of all kdr mutations.ConclusionsHere we provide the most-up-to-date survey on the geographic and temporal distribution of pyrethroid-predictive mutations in Ae. albopictus by combining kdr genotyping on current and historical samples with published data. While we confirm low levels of pyrethroid resistance in most analyzed samples, we find increasing frequencies of F1534C/S and V1016G in China and Greece or Italy, respectively. The observed patterns of kdr allele distribution support the hypothesis that on site emergence of resistance has contributed more than spread of resistance through mosquito migration/invasions to the current widespread of kdr alleles, emphasizing the importance of local surveillance programs and resistance management.

Project description:Current knowledge of the piRNA pathway is based mainly on studies on Drosophila melanogaster where three proteins of the Piwi subclade of the Argonaute family interact with PIWI-interacting RNAs to silence transposable elements in gonadal tissues. In mosquito species that transmit epidemic arboviruses such as dengue and chikungunya viruses, Piwi clade genes underwent expansion, are also expressed in the soma and cross-talk with proteins of recognized antiviral function cannot be excluded for some Piwi proteins. These observations underscore the importance of expanding our knowledge of the piRNA pathway beyond the model organism D. melanogaster. Here we focus on the emerging arboviral vector Aedes albopictus and we couple traditional approaches of expression and adaptive evolution analyses with most current computational predictions of protein structure to study evolutionary divergence among Piwi clade proteins. Superposition of protein homology models indicate possible high structure similarity among all Piwi proteins, with high levels of amino acid conservation in the inner regions devoted to RNA binding. On the contrary, solvent-exposed surfaces showed low conservation, with several sites under positive selection. Analysis of the expression profiles of Piwi transcripts during mosquito development and following infection with dengue serotype 1 or chikungunya viruses showed a concerted elicitation of all Piwi transcripts during viral dissemination of dengue viruses while maintenance of infection relied on expression of primarily Piwi5. Opposite, establishment of persistent infection by chikungunya virus is accompanied by increased expression of all Piwi genes, particularly Piwi4 and, again, Piwi5. Overall these results are consistent with functional specialization and a general antiviral role for Piwi5. Experimental evidences of sites under positive selection in Piwi1/3, Piwi4 and Piwi6, that have complex expression profiles, provide useful knowledge to design tailored functional experiments.

Project description:Glyphosate and glyphosate-based herbicides are among the most used chemicals in plant pest control. Both glyphosate and its main by-product Aminomethylphosphonic Acid (AMPA) are highly environmentally persistent and, through several processes (including surface runoff and bioaccumulation), affect species beyond their intended targets, especially in aquatic ecosystems. Aedes albopictus is a novel invasive arboviral vector in Colombia and has spread to much of the national territory in recent years. Strains of the bacterium Lysinibacillus sphaericus have shown the ability to degrade glyphosate into environmentally inert compounds, in addition to having great larvicidal efficiency in different mosquito species through the production of several proteins, including the surface layer (S-Layer) protein. The S-Layer is a bacterial structure consisting of glycoprotein monomers, and its functions are thought to include bacterial interactions, protection from the outside medium and biological control. The study assessed the entomopathogenic activity of L. sphaericus S-Layer protein on Ae. albopictus larvae, and the effects that glyphosate and its by-products have in this process. To that end, bioassays were performed to compare the larval mortality between different treatments with and without S-Layer, glyphosate, and glyphosate derivates. Comparisons were made through Analysis of variance (ANOVA) and Tukey's Honestly Significant Difference (HSD) analyses. Significant differences were found in larval mortality in the treatments, and larval mortality was greater when the S-Layer protein was present, though glyphosate field-doses (1.69 g/L) alone had a notable toxicity as well. An apparent synergic effect on the mortality of larval Ae. albopictus when exposed to mixtures containing 1500 ppm of the S-Layer protein, glyphosate, and/or glyphosate derivates was found. Further studies are needed for the in-depth understanding of this mechanism and its consequences on aquatic ecosystems.

Dataset Information

Improved reference genome of the arboviral vector Aedes albopictus.

Background

Results

Conclusion

Publications

Improved reference genome of the arboviral vector Aedes albopictus.

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