A photo zipper locked DNA nanomachine with an internal standard for precise miRNA imaging in living cells.

ABSTRACT: DNA nanomachines are capable of converting tiny triggers into autonomous accelerated cascade hybridization reactions and they have been used as a signal amplification strategy for intracellular imaging. However, the "always active" property of most DNA nanomachines with an "absolute intensity-dependent" signal acquisition mode results in "false positive signal amplification" by extracellular analytes and impairs detection accuracy. Here we design a photo zipper locked miRNA responsive DNA nanomachine (PZ-DNA nanomachine) based on upconversion nanoparticles (UCNPs) with a photo-cleavable DNA strand to block the miRNA recognition region, which provided sufficient protection to the DNA nanomachine against nonspecific extracellular activation and allowed satisfactory signal amplification for s

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC7448525 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature