Functional Expression and Characterization of Human Myristoylated-Arf1 in Nanodisc Membrane Mimetics.

ABSTRACT: Lipidated small GTP-binding proteins of the Arf family interact with multiple cellular partners and with membranes to regulate intracellular traffic and organelle structure. Here, we focus on the ADP-ribosylation factor 1 (Arf1), which interacts with numerous proteins in the Arf pathway, such as the ArfGAP ASAP1 that is highly expressed and activated in several cancer cell lines and associated with enhanced migration, invasiveness, and poor prognosis. Understanding the molecular and mechanistic details of Arf1 regulation at the membrane via structural and biophysical studies requires large quantities of fully functional protein bound to lipid bilayers. Here, we report on the production of a functional human Arf1 membrane platform on nanodiscs for biophysical studies. Large scale bacterial

SUBMITTER: Li Y 

PROVIDER: S-EPMC7448547 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature