Suppression of autophagy facilitates hydrogen gas-mediated lung cancer cell apoptosis.

ABSTRACT: Our previous study found that hydrogen gas (H₂) could efficiently inhibit lung cancer progression; however, the underlying mechanisms still remains to be elucidated. The present study aimed to explore the roles of H₂ in lung cancer cell autophagy, and reveal the effects of autophagy on H₂-mediated lung cancer cell apoptosis and the underlying mechanisms. The expression levels of proteins associated with cell apoptosis and autophagy were detected using western blot analysis. Cell autophagy was inhibited by 3-methyladenine treatment or Beclin1 downregulation, while rapamycin was used to induce autophagy. Cell growth and apoptosis were detected using the Cell Counting Kit-8 and flow cytometry assays, respectively. The results demonstrated that cell apoptosis a

SUBMITTER: Liu L 

PROVIDER: S-EPMC7448556 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature