Hi-C chromosome conformation capture sequencing of avian genomes using the BGISEQ-500 platform.

ABSTRACT:

Background

Hi-C experiments couple DNA-DNA proximity with next-generation sequencing to yield an unbiased description of genome-wide interactions. Previous methods describing Hi-C experiments have focused on the industry-standard Illumina sequencing. With new next-generation sequencing platforms such as BGISEQ-500 becoming more widely available, protocol adaptations to fit platform-specific requirements are useful to give increased choice to researchers who routinely generate sequencing data.

Results

We describe an in situ Hi-C protocol adapted to be compatible with the BGISEQ-500 high-throughput sequencing platform. Using zebra finch (Taeniopygia guttata) as a biological sample, we demonstrate how Hi-C libraries can be constructed to generate informative data using the BGIS

SUBMITTER: Sandoval-Velasco M 

PROVIDER: S-EPMC7448675 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature