The role of human serum and solution chemistry in fibrinogen peptide-nanoparticle interactions.

ABSTRACT: In living systems, the biomolecules that coat nanoparticles (NPs) alter the NP biological identity and response. Although some biomolecules are more effective in mediating NP stability or biological fate, it is difficult to monitor an individual biomolecule within the complexity of the biota. To understand the dependence of protein-NP interactions on common variations in blood, we have evaluated binding between silica NPs and a model gamma-fibrinogen (GF) peptide. Fibrinogen is commonly identified within the protein corona fingerprint of human serum, but its abundance on the NP varies. To assess the relative importance of human serum and solution conditions, GF peptide and silica NP interactions were evaluated with and without serum across pH, NaCl concentrations, and glucose concentration

SUBMITTER: Zapata A 

PROVIDER: S-EPMC7448706 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature