Genome-wide capture sequencing to detect hepatitis C virus at the end of antiviral therapy.

ABSTRACT:

Background

Viral relapse is a major concern in hepatitis C virus (HCV) antiviral therapy. Currently, there are no satisfactory methods to predict viral relapse, especially in the era of direct acting antivirals in which the virus often quickly becomes undetectable using PCR-based approaches that focus on a small viral region. Next-generation sequencing (NGS) provides an alternative option for viral detection in a genome-wide manner. However, owing to the overwhelming dominance of human genetic content in clinical specimens, direct detection of HCV by NGS has a low sensitivity and hence viral enrichment is required.

Methods

Based on template-dependent multiple displacement amplification (tdMDA), an improved method for whole genome amplification (Wang et al., 2017. Biotechniqu

SUBMITTER: Peng P 

PROVIDER: S-EPMC7448998 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature