Cell metabolomics to study the function mechanism of Cyperus rotundus L. on triple-negative breast cancer cells.

ABSTRACT:

Background

Triple-negative breast cancer (TNBC) is a kind of malignant tumor with higher recurrence and metastasis rate. According to historical records, the dry rhizomes Cyperus rotundus L. could be ground into powder and mixed with ginger juice and wine for external application for breast cancer. We studied the effect of the ethanol extract of Cyperus rotundus L. (EECR) on TNBC cells and found its' apoptosis-inducing effect with a dose-relationship. But the function mechanism of EECR on TNBC is still mysterious. Hence, the present research aimed to detect its function mechanism at the small molecule level through ultra-high performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) metabolomics.

Methods

The CCK-8 assay and

SUBMITTER: Ma S 

PROVIDER: S-EPMC7449030 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature