Novel Aminoglycoside-Tolerant Phoenix Colony Variants of Pseudomonas aeruginosa.

ABSTRACT: Pseudomonas aeruginosa is an opportunistic bacterial pathogen and is known to produce biofilms. We previously showed the emergence of colony variants in the presence of tobramycin-loaded calcium sulfate beads. In this study, we characterized the variant colonies, which survived the antibiotic treatment, and identified three distinct phenotypes-classically resistant colonies, viable but nonculturable colonies (VBNC), and phoenix colonies. Phoenix colonies, described here for the first time, grow out of the zone of clearance of antibiotic-loaded beads from lawn biofilms while there are still very high concentrations of antibiotic present, suggesting an antibiotic-resistant phenotype. However, upon subculturing of these isolates, phoenix colonies return to wild-type levels of antibiotic susce

SUBMITTER: Sindeldecker D 

PROVIDER: S-EPMC7449220 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature