Development of a Plasmid Shuttle Vector System for Genetic Manipulation of Chlamydia psittaci.

ABSTRACT: The obligate intracellular bacterium Chlamydia psittaci is a known avian pathogen causing psittacosis in birds and is capable of zoonotic transmission. In human pulmonary infections, C. psittaci can cause pneumonia associated with significant mortality if inadequately diagnosed and treated. Although intracellular C. psittaci manipulates host cell organelles for its replication and survival, it has been difficult to demonstrate host-pathogen interactions in C. psittaci infection due to the lack of easy-to-handle genetic manipulation tools. Here, we show the genetic transformation of C. psittaci using a plasmid shuttle vector that contains a controllable gene induction system. The 7,553-bp plasmid p01DC12 was prepared from the nonavian C. psittaci st

SUBMITTER: Shima K 

PROVIDER: S-EPMC7449628 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature