Adipose-derived mesenchymal stem cells promote the malignant phenotype of cervical cancer.

ABSTRACT: Epidemiological studies indicate that obesity negatively affects the progression and treatment of cervical-uterine cancer. Recent evidence shows that a subpopulation of adipose-derived stem cells can alter cancer properties. In the present project, we described for the first time the impact of adipose-derived stem cells over the malignant behavior of cervical cancer cells. The transcriptome of cancer cells cultured in the presence of stem cells was analyzed using RNA-seq. Changes in gene expression were validated using digital-PCR. Bioinformatics tools were used to identify the main transduction pathways disrupted in cancer cells due to the presence of stem cells. In vitro and in vivo assays were conducted to validate cellular and molecular processes altered in cervical cancer cells owing

SUBMITTER: Castro-Oropeza R 

PROVIDER: S-EPMC7450089 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature