In-cell infrared difference spectroscopy of LOV photoreceptors reveals structural responses to light altered in living cells.

ABSTRACT: Proteins are usually studied in well-defined buffer conditions, which differ substantially from those within a host cell. In some cases, the intracellular environment has an impact on the mechanism, which might be missed by in vitro experiments. IR difference spectroscopy previously has been applied to study the light-induced response of photoreceptors and photoenzymes in vitro Here, we established the in-cell IR difference (ICIRD) spectroscopy in the transmission and attenuated total reflection configuration to investigate the light-induced response of soluble proteins in living bacterial cells. ICIRD spectroscopy on the light, oxygen, or voltage (LOV) domains of the blue light receptors aureochrome and phototropin revealed a suppression of the response of specific secondary

SUBMITTER: Goett-Zink L 

PROVIDER: S-EPMC7450117 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature