Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development.

ABSTRACT: Neurogenesis processes differ in different areas of the cortex in many species, including humans. Here, we performed single-cell transcriptome profiling of the four cortical lobes and pons during human embryonic and fetal development. We identified distinct subtypes of neural progenitor cells (NPCs) and their molecular signatures, including a group of previously unidentified transient NPCs. We specified the neurogenesis path and molecular regulations of the human deep-layer, upper-layer, and mature neurons. Neurons showed clear spatial and temporal distinctions, while glial cells of different origins showed development patterns similar to those of mice, and we captured the developmental trajectory of oligodendrocyte lineage cells until the human mid-fetal stage. Additionally, we verified r

SUBMITTER: Fan X 

PROVIDER: S-EPMC7450478 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature