Automated pneumatic shuttle for magnetic field cycling and parahydrogen hyperpolarized multidimensional NMR.

ABSTRACT: We present a simple-to-implement pneumatic sample shuttle for automation of magnetic field cycling and multidimensional NMR. The shuttle system is robust allowing automation of hyperpolarized and non-hyperpolarized measurements, including variable field lifetime measurements, SABRE polarization optimization, and SABRE multidimensional experiments. Relaxation-protected singlet states are evaluated by variable-field T₁ and T_S measurements. Automated shuttling facilitates characterization of hyperpolarization dynamics, field dependence and polarization buildup rates. Furthermore, reproducible hyperpolarization levels at every shuttling event enables automated 2D hyperpolarized NMR, including the first inverse ¹⁵N/¹H HSQC. We uncover binding mechanis

SUBMITTER: TomHon P 

PROVIDER: S-EPMC7450533 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature