Performance of a universal PCR assay to identify different Leishmania species causative of Old World cutaneous leishmaniasis.

ABSTRACT:

Background

The characterization of Leishmania species is important for clinical management of the diseases and the epidemiological control of the parasite distribution. Most of the published polymerase chain reaction (PCR) amplification methods lack the ability to identify all species complexes, have low performance and usually need post-PCR procedures. There is a need for improving the diagnosis of CL by development of an accurate affordable PCR method to identify all Leishmania species in clinical specimens.

Methods

We designed an optimized a PCR amplifying the internal transcribed spacer 2 sequence of the ribosomal RNA gene (ITS2) aligned from different strains of CL-causing Leishmania species in the Old World. The performance of the method was evaluated on lesion samples

SUBMITTER: Nateghi Rostami M 

PROVIDER: S-EPMC7450935 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature