Saving time maintaining reliability: a new method for quantification of Tetranychus urticae damage in Arabidopsis whole rosettes.

ABSTRACT:

Background

The model species Tetranychus urticae produces important plant injury and economic losses in the field. The current accepted method for the quantification of the spider mite damage in Arabidopsis whole rosettes is time consuming and entails a bottleneck for large-scale studies such as mutant screening or quantitative genetic analyses. Here, we describe an improved version of the existing method by designing an automatic protocol. The accuracy, precision, reproducibility and concordance of the new enhanced approach are validated in two Arabidopsis accessions with opposite damage phenotypes. Results are compared to the currently available manual method.

Results

Image acquisition experiments revealed that the automatic settings plus 10 values of brightness and the bl

SUBMITTER: Ojeda-Martinez D 

PROVIDER: S-EPMC7450957 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature