Stable and selective permeable hydrogel microcapsules for high-throughput cell cultivation and enzymatic analysis.

ABSTRACT:

Background

Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows identification of catalysts with improved performance. However, emulsion droplet-based technologies prevent cell proliferation to high density and preclude the feasibility of biochemical reactions that require the exchange of small molecule substrates. Here, we report on the development of a high-throughput screening method that addresses these shortcomings and that relies on a novel selective permeable polymer hydrogel microcapsule.

Results

Hollow-core

SUBMITTER: Di Girolamo S 

PROVIDER: S-EPMC7451113 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature