Investigation of Twenty Metal, Metal Oxide, and Metal Sulfide Nanoparticles' Impact on Differentiated Caco-2 Monolayer Integrity.

ABSTRACT: The use of engineered nanomaterials (ENMs) in foods and consumer products is rising, increasing the potential for unintentional ingestion. While the cytotoxicity of many ENMs has been investigated, less attention has been given to adverse impact on the intestinal barrier integrity. Chronical disruption of gastrointestinal integrity can have far reaching health implications. Using fully differentiated Caco-2 cells, the perturbation of intestinal barrier function and cytotoxicity were investigated for 20 metal, metal oxide, and metal sulfide ENMs. Caco-2 cells were exposed to 50 μg/mL ENMs for 24 hours. ENM formulations were characterized at 0 and 24 hours, and In Vitro Sedimentation, Diffusion and Dosimetry Modeling was applied to calculate the effective dose of exposure during 24 hours. Th

SUBMITTER: Mortensen NP 

PROVIDER: S-EPMC7451203 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature