Anomalous Diffusion Characterization by Fourier Transform-FRAP with Patterned Illumination.

ABSTRACT: Fourier transform fluorescence recovery after photobleaching (FT-FRAP) with patterned illumination is theorized and demonstrated for quantitatively evaluating normal and anomalous diffusion. Diffusion characterization is routinely performed to assess mobility in cell biology, pharmacology, and food science. Conventional FRAP is noninvasive, has low sample volume requirements, and can rapidly measure diffusion over distances of a few micrometers. However, conventional point-bleach measurements are complicated by signal-to-noise limitations, the need for precise knowledge of the photobleach beam profile, potential for bias due to sample heterogeneity, and poor compatibility with multiphoton excitation because of local heating. In FT-FRAP with patterned illumination, the time-dependent fluore

SUBMITTER: Geiger AC 

PROVIDER: S-EPMC7451902 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature