GC-Content Dependence of Elastic and Overstretching Properties of DNA:RNA Hybrid Duplexes.

ABSTRACT: DNA:RNA hybrid duplex plays important roles in various biological processes. Both its structural stability and interactions with proteins are highly sequence dependent. In this study, we utilize homebuilt optical tweezers to investigate how GC contents in the sequence influence the structural and mechanical properties of DNA:RNA hybrid by measuring its contour length, elasticities, and overstretching dynamics. Our results support that the DNA:RNA hybrid adopts a conformation between the A- and B-form helix, and the GC content does not affect its structural and elastic parameters obviously when varying from 40 to 60% before the overstretching transition of DNA:RNA hybrid occurs. In the overstretching transition, however, our study unravels significant heterogeneity and strong sequence depen

SUBMITTER: Yang D 

PROVIDER: S-EPMC7451903 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature