In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species.

ABSTRACT: Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenic Leishmania spp. as well as hundreds of strains, but large-scale proteomics analyses did not follow these achievements and the Leishmania proteome remained mostly uncharacterized. Here, we report a comprehensive comparative study of the proteomes of strains representing L. braziliensis, L. panamensis and L. guyanensis species. Proteins extracted by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. "Total Protein Approach" and "

SUBMITTER: Pinho N 

PROVIDER: S-EPMC7451982 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature