Oxidation of HDAC4 by Nox4-derived H₂O₂ maintains tube formation by endothelial cells.

ABSTRACT: NADPH oxidases produce reactive oxygen species that differ in localization, type and concentration. Within the Nox family only Nox4 produces H₂O₂ which can directly oxidize cysteine residues. With this post-translational modification, activity, stability, localization and protein-protein interactions of the affected protein is altered. Nox4 controls differentiation, cellular homeostasis and prevents inflammation. Therefore, is likely that epigenetic mechanisms contribute to the effects of Nox4. One group of epigenetic modifiers are class IIa histone deacetylases (HDACs). We hypothesize that Nox4-derived H₂O₂ oxidizes HDACs and analyzed whether HDACs can be differentially oxidized by Nox4. As an artificial system, we utilized HEK293 cells, overexp

SUBMITTER: Schader T 

PROVIDER: S-EPMC7452117 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature