Tissue-in-a-Tube: three-dimensional in vitro tissue constructs with integrated multimodal environmental stimulation.

ABSTRACT: Three-dimensional (3D) in vitro tissue models are superior to two-dimensional (2D) cell cultures in replicating natural physiological/pathological conditions by recreating the cellular and cell-matrix interactions more faithfully. Nevertheless, current 3D models lack either the rich multicellular environment or fail to provide appropriate biophysical stimuli both of which are required to properly recapitulate the dynamic in vivo microenvironment of tissues and organs. Here, we describe the rapid construction of multicellular, tubular tissue constructs termed Tissue-in-a-Tube using self-assembly process in tubular molds with the ability to incorporate a variety of biophysical stimuli such as electrical field, mechanical deformation, and shear force of the fluid flow. Unlike ot

SUBMITTER: Shahin-Shamsabadi A 

PROVIDER: S-EPMC7452320 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature