Challenges to Differentiate Hepatitis C Genotype 1 and 6: Results from A Field-Study in Cambodia.

ABSTRACT:

Introduction

We aim to report on results and challenges of different methods used for hepatitis C (HCV) genotyping in a Cambodian HCV/HIV coinfection project.

Methods

Samples of 106 patients were available. HCV genotyping was initially (63 samples) done by the LightPower Taqman real-time PCR method (Viet A Corp.) and quality controlled using the Versant 2.0 line probe assay (Siemens Healthcare). Next, following interim quality control results, all 106 samples were (re)genotyped with Versant 2.0, complemented with 5'UTR/core sequencing for uninterpretable/incomplete Versant results.

Results

Using Versant, 103 (97.2%) of the 106 HCV-coinfected patients had an interpretable genotype result: 1b (50.5%), 6 non-a/non-b (30.1%), 1a (6.8%), 6a or b (4.9%), 2 (3.9%), 1 (2.9%)

SUBMITTER: De Weggheleire A 

PROVIDER: S-EPMC7452997 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature