Inhibition of lncRNA TMPO‑AS1 suppresses proliferation, migration and invasion of colorectal cancer cells by targeting miR‑143‑3p.

ABSTRACT: Long non‑coding RNAs (lncRNAs) are widely studied in cancer pathogenesis. Accumulating evidence has demonstrated that lncRNAs are involved in the cellular progression of colorectal cancer (CRC). However, the regulatory mechanism of lncRNA TMPO‑antisense (AS)1 in CRC has not been fully elucidated. The present study aimed to elucidate the role and regulatory mechanisms of lncRNA TMPO‑AS1 in CRC. In the present study, the expression levels of TMPO‑AS1 and microRNA‑143‑3p (miR‑143‑3p) were detected using reverse transcription‑quantitative PCR assay. The relative protein expression levels were measured via western blot analysis. MTT and Transwell assays were used to determine cell proliferation, migration and invasion, while a luciferase reporter assay was performed to assess the relationship b

SUBMITTER: Zhao L 

PROVIDER: S-EPMC7453500 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature