Clinically relevant radioresistant rhabdomyosarcoma cell lines: functional, molecular and immune-related characterization.

ABSTRACT:

Background

The probability of local tumor control after radiotherapy (RT) remains still miserably poor in pediatric rhabdomyosarcoma (RMS). Thus, understanding the molecular mechanisms responsible of tumor relapse is essential to identify personalized RT-based strategies. Contrary to what has been done so far, a correct characterization of cellular radioresistance should be performed comparing radioresistant and radiosensitive cells with the same isogenic background.

Methods

Clinically relevant radioresistant (RR) embryonal (RD) and alveolar (RH30) RMS cell lines have been developed by irradiating them with clinical-like hypo-fractionated schedule. RMS-RR cells were compared to parental isogenic counterpart (RMS-PR) and studied following the radiobiological concept of the "6

SUBMITTER: Petragnano F 

PROVIDER: S-EPMC7453562 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature