Ictal onset sites and γ-aminobutyric acidergic neuron loss in epileptic pilocarpine-treated rats.

ABSTRACT:

Objective

The present study tested whether ictal onset sites are regions of more severe interneuron loss in epileptic pilocarpine-treated rats, a model of human temporal lobe epilepsy.

Methods

Local field potential recordings were evaluated to identify ictal onset sites. Electrode sites were visualized in Nissl-stained sections. Adjacent sections were processed with proximity ligation in situ hybridization for glutamic acid decarboxylase 2 (Gad2). Gad2 neuron profile numbers at ictal onset sites were compared to contralateral regions. Other sections were processed with immunocytochemistry for reelin or nitric oxide synthase (NOS), which labeled major subtypes of granule cell layer-associated interneurons. Stereology was used to estimate numbers of reelin and NOS granule cell

SUBMITTER: Wyeth M 

PROVIDER: S-EPMC7453688 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature