Drivers of recombinant soluble influenza A virus hemagglutinin and neuraminidase expression in mammalian cells.

ABSTRACT: Recombinant soluble trimeric influenza A virus hemagglutinins (HA) and tetrameric neuraminidases (NAs) have proven to be excellent tools to decipher biological properties. Receptor binding and sialic acid cleavage by recombinant proteins correlate satisfactorily compared to whole viruses. Expression of HA and NA can be achieved in a plethora of different laboratory hosts. For immunological and receptor interaction studies however, insect and mammalian cell expressed proteins are preferred due to the presence of N-linked glycosylation and disulfide bond formation. Because mammalian-cell expression is widely applied, an increased expression yield is an important goal. Here we report that using codon-optimized genes and sfGFP fusions, the expression yield of HA can be significantly improved.

SUBMITTER: van der Woude R 

PROVIDER: S-EPMC7454420 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature