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Reliability of Self-Sampling for Accurate Assessment of Respiratory Virus Viral and Immunologic Kinetics.

ABSTRACT: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic demonstrates the need for accurate and convenient approaches to diagnose and therapeutically monitor respiratory viral infections. We demonstrated that self-sampling with mid-nasal foam swabs is well-tolerated and provides quantitative viral output concordant with flocked swabs. Using longitudinal home-based self-sampling, we demonstrate that nasal cytokine levels correlate and cluster according to immune cell of origin. Periods of stable viral loads are followed by rapid elimination, which could be coupled with cytokine expansion and contraction. Nasal foam swab self-sampling at home provides a precise, mechanistic readout of respiratory virus shedding and local immune responses.

SUBMITTER: Waghmare A

PROVIDER: S-EPMC7454707 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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Reliability of Self-Sampling for Accurate Assessment of Respiratory Virus Viral and Immunologic Kinetics.

Waghmare Alpana A Krantz Elizabeth M EM Baral Subhasish S Vasquez Emma E Loeffelholz Tillie T Chung E Lisa EL Pandey Urvashi U Kuypers Jane J Duke Elizabeth R ER Jerome Keith R KR Greninger Alexander L AL Reeves Daniel B DB Hladik Florian F Cardozo-Ojeda E Fabian EF Boeckh Michael M Schiffer Joshua T JT

The Journal of infectious diseases 20220801 2

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic demonstrates the need for accurate and convenient approaches to diagnose and therapeutically monitor respiratory viral infections. We demonstrated that self-sampling with mid-nasal foam swabs is well-tolerated and provides quantitative viral output concordant with flocked swabs. Using longitudinal home-based self-sampling, we demonstrate that nasal cytokine levels correlate and cluster according to immune cell of origin. P ...[more]

PMID: 32710762

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Dataset Information

Reliability of Self-Sampling for Accurate Assessment of Respiratory Virus Viral and Immunologic Kinetics.

Publications

Reliability of Self-Sampling for Accurate Assessment of Respiratory Virus Viral and Immunologic Kinetics.

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