Implementing cell-free DNA of pancreatic cancer patient-derived organoids for personalized oncology.

ABSTRACT: One of the major challenges in using pancreatic cancer patient-derived organoids (PDOs) in precision oncology is the time from biopsy to functional characterization. This is particularly true for endoscopic ultrasound-guided fine-needle aspiration biopsies, typically resulting in specimens with limited tumor cell yield. Here, we tested conditioned media of individual PDOs for cell-free DNA to detect driver mutations already early on during the expansion process to accelerate the genetic characterization of PDOs as well as subsequent functional testing. Importantly, genetic alterations detected in the PDO supernatant, collected as early as 72 hours after biopsy, recapitulate the mutational profile of the primary tumor, indicating suitability of this approach to subject PDOs to drug testing

SUBMITTER: Dantes Z 

PROVIDER: S-EPMC7455062 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature