Coordinated regulation of Rel expression by MAP3K4, CBP, and HDAC6 controls phenotypic switching.

ABSTRACT: Coordinated gene expression is required for phenotypic switching between epithelial and mesenchymal phenotypes during normal development and in disease states. Trophoblast stem (TS) cells undergo epithelial-mesenchymal transition (EMT) during implantation and placentation. Mechanisms coordinating gene expression during these processes are poorly understood. We have previously demonstrated that MAP3K4-regulated chromatin modifiers CBP and HDAC6 each regulate thousands of genes during EMT in TS cells. Here we show that CBP and HDAC6 coordinate expression of only 183 genes predicted to be critical regulators of phenotypic switching. The highest-ranking co-regulated gene is the NF-κB family member Rel. Although NF-κB is primarily regulated post-transcriptionally, CBP and HDAC6 control Rel tran

SUBMITTER: Shendy NAM 

PROVIDER: S-EPMC7455715 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature