Cholesterol 25-hydroxylase protects against experimental colitis in mice by modulating epithelial gut barrier function.

ABSTRACT: Cholesterol 25-hydroxylase (CH25H) encodes the enzyme that converts cholesterol to 25-hydroxycholesterol (25-HC). 25-HC has been demonstrated to be involved in the pathogenesis of inflammatory bowel disease. However, the role of CH25H in experimental colitis remains unknown. Dextran sulfate sodium (DSS)-induced colitis was monitored in wild type and Ch25h^-/- mice in 8-week-old male for 7 days by assessment of body weight, histology, inflammatory cellular infiltration, and colon length. The function of CH25H was investigated using loss-of-function and gain-of-function such as Ch25h-deficient mice, supplementation with exogenous 25-HC and treatment of 25-HC into Caco2 and HCT116 colonic epithelial cells. Ch25h^-/- mice with DSS-induced colitis exhibited aggravated injury

SUBMITTER: Sheng N 

PROVIDER: S-EPMC7455728 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature