Development of syngeneic murine cell lines for use in immunocompetent orthotopic lung cancer models.

ABSTRACT:

Background

Immunocompetent animal models are required to study tumor-host interactions, immunotherapy, and immunotherapeutic combinations, however the currently available immunocompetent lung cancer models have substantial limitations. While orthotopic models potentially help fill this gap, the utility of these models has been limited by the very small number of murine lung cancer cell lines capable of forming orthotopic tumors in immunocompetent C57BL/6 hosts.

Methods

Primary lung tumors with specific genetic alterations were created in C57BL/6 background mice. These tumors were then passaged through other animals to increase tumorigenicity and select for the ability to grow in a non-self animal. Once tumors demonstrated growth in a non-self host, cell lines were establishe

SUBMITTER: Nolan K 

PROVIDER: S-EPMC7455907 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature