Insights into the Autoproteolytic Processing and Catalytic Mechanism of the Chlamydia trachomatis Virulence-Associated Protease CPAF.

ABSTRACT: CPAF (chlamydial protease-like activity factor) is a Chlamydia trachomatis protease that is translocated into the host cytosol during infection. CPAF activity results in dampened host inflammation signaling, cytoskeletal remodeling, and suppressed neutrophil activation. Although CPAF is an emerging antivirulence target, its catalytic mechanism has been unexplored to date. Steady state kinetic parameters were obtained for recombinant CPAF with vimentin-derived peptide substrates using a high-performance liquid chromatography-based discontinuous assay (k_cat = 45 ± 0.6 s^-1; k_cat/K_m = 0.37 ± 0.02 μM^-1 s^-1) or a new fluorescence-based continuous assay (k_cat = 23 ± 0.7 s^-1;

SUBMITTER: Dudiak BM 

PROVIDER: S-EPMC7455923 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature