Multicolor 3D MINFLUX nanoscopy of mitochondrial MICOS proteins.

ABSTRACT: The mitochondrial contact site and cristae organizing system (MICOS) is a multisubunit protein complex that is essential for the proper architecture of the mitochondrial inner membrane. MICOS plays a key role in establishing and maintaining crista junctions, tubular or slit-like structures that connect the cristae membrane with the inner boundary membrane, thereby ensuring a contiguous inner membrane. MICOS is enriched at crista junctions, but the detailed distribution of its subunits around crista junctions is unclear because such small length scales are inaccessible with established fluorescence microscopy. By targeting individually activated fluorophores with an excitation beam featuring a central zero-intensity point, the nanoscopy method called MINFLUX delivers single-digit nanometer-

SUBMITTER: Pape JK 

PROVIDER: S-EPMC7456099 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature