Syntaxin 3 is essential for photoreceptor outer segment protein trafficking and survival.

ABSTRACT: Trafficking of photoreceptor membrane proteins from their site of synthesis in the inner segment (IS) to the outer segment (OS) is critical for photoreceptor function and vision. Here we evaluate the role of syntaxin 3 (STX3), in trafficking of OS membrane proteins such as peripherin 2 (PRPH2) and rhodopsin. Photoreceptor-specific Stx3 knockouts [Stx3 ^f/f(iCre75) and Stx3 ^f/f(CRX-Cre) ] exhibited rapid, early-onset photoreceptor degeneration and functional decline characterized by structural defects in IS, OS, and synaptic terminals. Critically, in the absence of STX3, OS proteins such as PRPH2, the PRPH2 binding partner, rod outer segment membrane protein 1 (ROM1), and rhodopsin were mislocalized along the microtubules to the IS, ce

SUBMITTER: Kakakhel M 

PROVIDER: S-EPMC7456102 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature