Revealing the mechanism of repressor inactivation during switching of a temperate bacteriophage.

ABSTRACT: Temperate bacteriophages can enter one of two life cycles following infection of a sensitive host: the lysogenic or the lytic life cycle. The choice between the two alternative life cycles is dependent upon a tight regulation of promoters and their cognate regulatory proteins within the phage genome. We investigated the genetic switch of TP901-1, a bacteriophage of Lactococcus lactis, controlled by the CI repressor and the modulator of repression (MOR) antirepressor and their interactions with DNA. We determined the solution structure of MOR, and we solved the crystal structure of MOR in complex with the N-terminal domain of CI, revealing the structural basis of MOR inhibition of CI binding to the DNA operator sites. ¹⁵N NMR Carr-Purcell-Meiboom-Gill (CPMG) relaxation dis

SUBMITTER: Rasmussen KK 

PROVIDER: S-EPMC7456139 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature