The histone methyltransferase DOT1L prevents antigen-independent differentiation and safeguards epigenetic identity of CD8⁺ T cells.

ABSTRACT: Cytotoxic T cell differentiation is guided by epigenome adaptations, but how epigenetic mechanisms control lymphocyte development has not been well defined. Here we show that the histone methyltransferase DOT1L, which marks the nucleosome core on active genes, safeguards normal differentiation of CD8⁺ T cells. T cell-specific ablation of Dot1L resulted in loss of naïve CD8⁺ T cells and premature differentiation toward a memory-like state, independent of antigen exposure and in a cell-intrinsic manner. Mechanistically, DOT1L controlled CD8⁺ T cell differentiation by ensuring normal T cell receptor density and signaling. DOT1L also maintained epigenetic identity, in part by indirectly supporting the repression of developmentally regulated genes. Finall

SUBMITTER: Kwesi-Maliepaard EM 

PROVIDER: S-EPMC7456197 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature