Analyzing and validating the prognostic value and mechanism of colon cancer immune microenvironment.

ABSTRACT:

Background

Colon cancer is a disease with high malignancy and incidence in the world. Tumor immune microenvironment (TIM) and tumor mutational burden (TMB) have been proved to play crucial roles in predicting clinical outcomes and therapeutic efficacy, but the correlation between them and the underlying mechanism were not completely understood in colon cancer.

Methods

In this study, we used Single-Sample Gene Set Enrichment Analysis (ssGSEA) and unsupervised consensus clustering analysis to divide patients from the TCGA cohort into three immune subgroups. Then we validated their differences in immune cell infiltration, overall survival outcomes, clinical phenotypes and expression levels of HLA and checkpoint genes by Mann-Whitney tests. We performed weighted correlation netw

SUBMITTER: Wang X 

PROVIDER: S-EPMC7456375 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature