Efficient acute and chronic infection of stem cell-derived hepatocytes by hepatitis C virus.

ABSTRACT:

Objective and design

Human stem cell-derived hepatocyte-like cells (HLCs) have shown high potential as authentic model for dissection of the HCV life cycle and virus-induced pathogenesis. However, modest HCV replication, possibly due to robust innate immune responses, limits their broader use. To overcome these limitations and to dissect the mechanisms responsible for control of HCV, we analysed expression of key components of the interferon (IFN) system in HLCs, assessed permissiveness for different HCV strains and blocked innate immune signalling by pharmacological intervention.

Results

Transcriptional profiling revealed that HLCs constitutively express messenger RNA of RLRs, and members of the IFN pathway. Moreover, HLCs upregulated IFNs and canonical interferon-regulated

SUBMITTER: Carpentier A 

PROVIDER: S-EPMC7456736 | biostudies-literature | 2020 Sep

REPOSITORIES: biostudies-literature